Firstly, paste your complex file in PDB format example input; example output

or upload the PDB file from your local machine. Click here to download the example file.
Secondly, please input the correct parameters:
PDB ID: eg(DNA mutation): 1MSE; eg(protein mutation): 1AAY
Chain ID: eg(DNA mutation): A; eg(protein mutation): A
Mutation: eg(DNA mutation): AT5CG; eg(protein mutation): D120A

Thirdly, choose appropriate mutation type according to your data:
     

Citation: PNBACE: an ensemble algorithm to predict the effects of mutations on protein-nucleic acid binding affinity.